mwm molecular weight marker  (Bio-Rad)

Journal: bioRxiv

Article Title: Gel Electrophoresis/Electroelution Sorting Fractionator combined with Filter Aided Sample preparation (FASP) for deep proteomic analysis

doi: 10.1101/2021.04.16.440150

Figure Lengend Snippet: Reproducibility achieved with the SDS-PAGE-based GEES fractionator with automatic or programmed collection. A and B are analytical SDS-PAGE of the collected fractions (A): Three replicates of the Bio-Rad Low Molecular Weight Marker fractionation carried out on different days. (B): Three replicates of the E. coli Tris soluble protein fractionation carried out on different days. MWM: Molecular Weight Marker. US: Unfractionated sample.

Article Snippet: Two samples were used for the reproducibility evaluation: the Bio-Rad Low Molecular Weight Markers (MWM) and the E. coli proteome.

Techniques: SDS Page, Molecular Weight, Marker, Fractionation

Journal: bioRxiv

Article Title: Gel Electrophoresis/Electroelution Sorting Fractionator combined with Filter Aided Sample preparation (FASP) for deep proteomic analysis

doi: 10.1101/2021.04.16.440150

Figure Lengend Snippet: Loading capacity of the SDS-PAGE-based GEES fractionator. Fractions F1-F7 of the E. coli Tris soluble protein GEES fractionation, 250 µg in 50 µL (A), 1 mg in 200 µL (B), 1.5 mg in 100 µL (C), 1.5 mg in 300 µL (D). Brackets indicate protein dispersion among fractions F1 and F2. MWM: Molecular Weight Marker. US: Unfractionated sample.

Article Snippet: Two samples were used for the reproducibility evaluation: the Bio-Rad Low Molecular Weight Markers (MWM) and the E. coli proteome.

Techniques: SDS Page, Fractionation, Molecular Weight, Marker

Journal: bioRxiv

Article Title: Gel Electrophoresis/Electroelution Sorting Fractionator combined with Filter Aided Sample preparation (FASP) for deep proteomic analysis

doi: 10.1101/2021.04.16.440150

Figure Lengend Snippet: GEES fractionator-based analysis of the CaSki proteome. (A): SDS-PAGE/silver stain analysis of the protein fractions obtained after the SDS-PAGE-based GEES fractionation of 150 µg of the CaSki proteome. F1-12: fractions 1-12 (a volume corresponding to 1/25 of the fraction volume was analyzed), MWM: Molecular Weight Markers. (B): Normalized Molecular Weight distribution of the identified proteins using the GEES fractionator/MED-FASP approach (blue) or the MED-FASP method of the unfractionated sample (red). (C): Frequency per fraction of the protein molecular mass identified in only one fraction (post-translational modifications were not considered for protein mass calculation). (D): Cumulative identified proteins (blue) and peptides (red) counts for the GEES fractionator/MED-FASP approach. (E): Frequency per fraction of the peptide valence to mass ratio, of the unmodified peptides identified in only one fraction. (F): Cumulative identified proteins (blue) and peptides (red) counts for the FASP/GEES fractionator approach.

Article Snippet: Two samples were used for the reproducibility evaluation: the Bio-Rad Low Molecular Weight Markers (MWM) and the E. coli proteome.

Techniques: SDS Page, Silver Staining, Fractionation, Molecular Weight

Journal: bioRxiv

Article Title: Proteomic profiling of Mycobacterium tuberculosis culture filtrate identifies novel O-glycosylated proteins

doi: 10.1101/740134

Figure Lengend Snippet: (A) M. tuberculosis CFP analysis by 1D SDS-PAGE 15% and silver nitrate staining. Two different batches (lanes 1 and 2, 1.8 and 2.1 ug, respectively) and a composed and concentrated sample of both batches (lane 3, 12 ug) were loaded. Bands selected for MALDI-TOF/TOF mass spectrometry are indicated as H1, H2, H3 and H4. MWM: Molecular weight marker (Thermo Fischer Scientific, # 26616). (B) M. tuberculosis CFP analysis by 2D electrophoresis and silver nitrate staining. M. tuberculosis CFP composed sample (50 ug) was loaded. Immunoreactive spots selected for MALDI-TOF/TOF mass spectrometry are indicated with numbers. MWM: Molecular weight marker (Thermo Fischer Scientific, # 26616). (C) Western blot analysis of M. tuberculosis CPF. 2D gel performed equally as (B) was transferred to Protran 0.45 uM NC (GE Healtcare) and probed with rabbit anti-CFP antibody. Immunoreactive zones are indicated with a rectangle in the corresponding 2D gel.

Article Snippet: MWM: Molecular weight marker (Thermo Fischer Scientific, # 26616).

Techniques: SDS Page, Staining, Mass Spectrometry, Molecular Weight, Marker, Two-Dimensional Gel Electrophoresis, Western Blot